DNA

Part:BBa_K2100020:Design

Designed by: Kathleen Brandes   Group: iGEM16_MIT   (2016-10-14)


pENTR GAL4-VP16


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 723
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 723
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 723
    Illegal BamHI site found at 1
    Illegal XhoI site found at 477
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 723
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 723
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This basic part entry vector is flanked by L1 and L2 sites, which are used to denote a gene. This can be cascade with a promoter (flanked by L4, R1 sites) using an LR reaction and cloning into a cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.



Source

This is a fusion protein where GAL4 is derived from yeast and VP16 is from the herpes simplex virus.

References