DNA
Part:BBa_K2100020:Design
Designed by: Kathleen Brandes Group: iGEM16_MIT (2016-10-14)
pENTR GAL4-VP16
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 723
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 723
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 723
Illegal BamHI site found at 1
Illegal XhoI site found at 477 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 723
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 723
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This basic part entry vector is flanked by L1 and L2 sites, which are used to denote a gene. This can be cascade with a promoter (flanked by L4, R1 sites) using an LR reaction and cloning into a cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.
Source
This is a fusion protein where GAL4 is derived from yeast and VP16 is from the herpes simplex virus.